• Biomedical
• Natural Polymers for 3D Printing
• Material Science
• Biochemistry
• Cell Culture
• Tissue Scaffolds
• Sponge Structures
• Coatings
• Films and Sheets
• For research and development purposes only.

• Weigh the desired amount of BUGA-FibriCoL fibrillar collagen product and transfer it into an appropriately sized container after determining its concentration.
• Prepare a 0.01 M hydrochloric acid or 0.5 M acetic acid solution according to the volume you will work with after weighing the lyophilized product.
• Mix the lyophilized product with the prepared acid solution in a suitable mixing container (e.g., borosilicate beaker or bottle) at a low RPM (preferably 350-500 RPM) until homogeneous distribution is observed under magnetic stirring.
• If the prepared solution will not be used immediately, store it at +4 °C in a refrigerator.

Note: Freezing the dissolved product is not recommended.
NOTE: Fibrillar collagen inherently has fibrils, so full dissolution is not observed. However, it demonstrates homogeneous distribution within the solvent. Visible fibers in the solution are normal and do not pose a problem.
For Coating Procedure;
• Prepare the solution form of BUGA-FibriCoL as described above.
• Dilute the collagen to the desired concentration using sterile 0.01 N hydrochloric acid solution. Mix gently until the content is fully homogenized.
• Ensure the working concentration is suitable for fibrillar collagen preparation. This concentration should be determined based on specific application needs.
• Adjust the pH value carefully using sterile 0.1 M sodium hydroxide solution until the desired concentration and viscosity are achieved. Monitor the pH value closely (using a pH meter, phenol red, or pH paper).
• Store the prepared fibrillar collagen solution in sterile tubes, ready for application.