BUGA-Silk Fibroin Solutions, Sterile Filtrated, 50 mg/ml

Product Info

S1902


 

BUGA-Silk Fibroin Solutions, Sterile Filtrated, 50 mg/ml (BUGA-SF Solutions) contain fibroin protein, the basic building block of the silkworm cocoon. BUGA-SF Solutions have significant potential in medical applications due to their highly biocompatible and non-immune response-inducing properties. After the silk fibers are dissolved in the liquid fibroin solution, this solution can be used as an additive in culture or to create 3D scaffolds in tissue engineering studies. BUGA-SF Solutions consists of a light chain (Mw ~26 kDa) and a heavy chain (Mw ~390 kDa) linked by a disulfide bond. Its molecular weight is in the average range of 100 kDa. BUGA-SF Solutions has many positive intrinsic properties including shiny appearance, smooth texture, high biocompatibility and biodegradability, versatile processability, ease of functionalization, thermal stability, etc. and has properties that increase mechanical strength.

 

 

 

 

 

 

 

• Coatings
• Films and electrospun fibers
• Sponges
• Hydrogels
• 3D Tissue Scaffolds
• Tissue Engineering
• Medical Devices

• BUGA-SF Solutions is a light yellow solution with a concentration of 50 mg/ml, which can be diluted to the desired concentration using pure water.
• Store at 2-8 ℃ as it is protein-based.
• Avoid frequent freezing and thawing cycles, as this can cause cross-linking in the product, leading to the loss of its soluble form.
• If not used for an extended period, store in aliquots at -20 or -70℃.
• Thaw the product under controlled conditions at +4 ℃.
• Mix the solution gently by light movements; avoid vortexing, shaking, or vigorous pipetting, as these actions may cause cross-linking.
• Store at +4 ℃ throughout its shelf life.
Recommendation: To prevent protein aggregation, do not leave the product dissolved for more than one week.

 

Product Technical Specifications

Appearance

Transparent, cream

SmellNo data available

Packing quantity

20 ml-50 ml
Storage Temperature2-8 ºC
Shelf lifeMaximum 3 weeks from date of production
Concentration~50 mg/mL
Fibroin purity- SDS Page electrophoresis
Characteristic
Molecular Weight (kDa)Average mol. weight 100 kDa
SourceBombyx mori silkworm

 

• DeBari, M. K., King III, C. I., Altgold, T. A., & Abbott, R. D. (2021). Silk fibroin as a green material. ACS Biomaterials Science & Engineering, 7(8), 3530-3544.
• Sun, W., Gregory, D. A., Tomeh, M. A., & Zhao, X. (2021). Silk fibroin as a functional biomaterial for tissue engineering. International Journal of Molecular Sciences, 22(3), 1499.
• Narita, C., Okahisa, Y., Wataoka, I., & Yamada, K. (2020). Characterization of ground silk fibroin through comparison of nanofibroin and higher order structures. ACS omega, 5(36), 22786-22792.
• Dadras Chomachayi, M., Solouk, A., & Mirzadeh, H. (2019). Improvement of the electrospinnability of silk fibroin solution by atmospheric pressure plasma treatment. Fibers and Polymers, 20, 1594-1600.
• Rizzo, G., Lo Presti, M., Giannini, C., Sibillano, T., Milella, A., Matzeu, G., ... & Farinola, G. M. (2020). Silk fibroin processing from CeCl3 aqueous solution: fibers regeneration and doping with Ce (III). Macromolecular Chemistry and Physics, 221(13), 2000066.
• Rnjak‐Kovacina, J., DesRochers, T. M., Burke, K. A., & Kaplan, D. L. (2015). The effect of sterilization on silk fibroin biomaterial properties. Macromolecular bioscience, 15(6), 861-874.
• Zhang, W., Chen, L., Chen, J., Wang, L., Gui, X., Ran, J., ... & Zou, X. (2017). Silk fibroin biomaterial shows safe and effective wound healing in animal models and a randomized controlled clinical trial. Advanced healthcare materials, 6(10), 1700121.
• Bucciarelli, A., & Motta, A. (2022). Use of Bombyx mori silk fibroin in tissue engineering: From cocoons to medical devices, challenges, and future perspectives. Biomaterials Advances, 139, 212982.
• Lu, S., Wang, P., Zhang, F., Zhou, X., Zuo, B., You, X., ... & Tang, H. (2015). A novel silk fibroin nanofibrous membrane for guided bone regeneration: a study in rat calvarial defects. American journal of translational research, 7(11), 2244.
• Lujerdean, C., Baci, G. M., Cucu, A. A., & Dezmirean, D. S. (2022). The contribution of silk fibroin in biomedical engineering. Insects, 13(3), 286.

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