BUGA-Hyaluronic Acid from Rooster Comb
Product Info
BUGAMED Biotechnology produces hyaluronic acid, which is in the glycosaminoglycan group, from rooster combs with high purity. TS EN ISO standards are applied in the production of BUGA-Hyaluronic Acid from Rooster Comb (BUGA-HYA-RC) from rooster combs. In this context, within the scope of MDR and medical device regulations, TS EN ISO 10993-10, 17 and 18 and biocompatibility tests ISO 10993-5 standards are followed. BUGA-HYA-RC obtained from rooster combs has a high molecular weight (generally 2-4 MDa). In this direction, with its viscoelastic structure that can show both viscous and elastic properties, water retention capacity, and superior mechanical properties, injectable HA stands out with its use in intra-articular injections, dermal filler products and wound dressing material design processes.
• Biomedical
• Natural polymer for 3D printing
• Material Science
• Biochemistry and Cell culture studies
• Cosmetic creams and moisturizers
• Dermal filler products
• Osteoarthritis treatments
• Weigh BUGA-HYA-RC according to the desired concentration and transfer it to a mixing container.
• Depending on the solution you are using, add the measured HA to pure water, Sodium hydroxide solution, or PBS, and stir with a mechanical or magnetic stirrer until fully dissolved.
• To accelerate dissolution, keep the mixture at a temperature of 50°C.
• Continue with the same solvent used for diluting to the appropriate volume.
• For cross-linking studies, first activate the cross-linking agent with the solvent, then add BUGA-HYA-RC and mix for the optimal duration to achieve cross-linking.
• The temperature used to activate the cross-linking agent should be adjusted according to the activation degree of the agent.
• Once prepared, store the product at +4°C or room temperature.
• Homogenization is not recommended for highly concentrated solutions.
• Sodium hydroxide solution is recommended for studies requiring higher concentrations.
Product Technical Specifications
Appearance | Lyophilized cream color |
Smell | No data available |
Form | Sponge |
Storage temperature | -20 °C |
Shelf life | 2 years from date of production |
Resolution | Pure water, PBS or 0.1 M NaOH solution |
Molecular weight | 2-4 MDa |
HA source | Sourced from rooster comb |
Protein by Lowry | ≤ 2 % |
Endotoxin/LAL | ≤ 5 EU/ml |
Protein amount | ≤ %0,1 |
Nucleic acid amount | ≤ %0,01 |
Total amount of DNA and RNA | <4,0 mg/ml |
Amount of fat | 0 |
• Kang, D. Y., Kim, W. S., Heo, I. S., Park, Y. H., & Lee, S. (2010). Extraction of hyaluronic acid (HA) from rooster comb and characterization using flow field‐flow fractionation (FlFFF) coupled with multiangle light scattering (MALS). Journal of separation science, 33(22), 3530-3536. • Saranraj, P., & Naidu, M. A. (2013). Hyaluronic acid production and its applications-a review. Int J Pharm Biol Arch, 4(5), 853-59. • Kulkarni, S. S., Patil, S. D., & Chavan, D. G. (2018). Extraction, purification and characterization of hyaluronic acid from Rooster comb. Journal of Applied and Natural Science, 10(1), 313-315. • Graciela, C. Q., José Juan, E. C., Gieraldin, C. L., Xóchitl Alejandra, P. M., & Gabriel, A. Á. (2023). Hyaluronic Acid—Extraction Methods, Sources and Applications. Polymers, 15(16), 3473. • Kalkandelen, C., Su, S., Saatçioğlu, E., & Gündüz, O. (2020, November). Hyaluronic Acid Production and Analysis from Rooster Comb. In 2020 Medical Technologies Congress (TIPTEKNO) (pp. 1-4). IEEE. • Tabari Shahandasht, N., Bolandi, M., & Rahmati, M. (2022). Comparison of physicochemical and rheological characteristics of hyaluronic acid extracted from rooster comb using different methods. Journal of food science and technology (Iran), 19(127), 79-89. • Kang, D. Y., Kim, W. S., Heo, I. S., Park, Y. H., & Lee, S. (2010). Extraction of hyaluronic acid (HA) from rooster comb and characterization using flow field‐flow fractionation (FlFFF) coupled with multiangle light scattering (MALS). Journal of separation science, 33(22), 3530-3536. • Galvez-Martin, P., Soto-Fernandez, C., Romero-Rueda, J., Cabañas, J., Torrent, A., Castells, G., & Martinez-Puig, D. (2023). A novel hyaluronic acid matrix ingredient with regenerative, anti-aging and antioxidant capacity. International journal of molecular sciences, 24(5), 4774. • Chon, J. W., Kim, B., Seo, K. H., Bae, D., Jeong, D., & Song, K. Y. (2020). Physiochemical and Organoleptic Properties of Kefir Containing Different Concentrations of Hyaluronic Acid: A Preliminary Study. Journal of Dairy Science and Biotechnology, 38(3), 146-153. • Ayaki, M., Wakayama, S., & Yonei, Y. (2022). Effects of rooster comb degradation product containing low-molecular hyaluronic acid (INJUV) in individuals with eye dryness; open-label tr. ial with no control group. Glycative Stress Research, 9(2), 97-105.
