BUGA-CoL Type I Atelocollagen Insoluble Fibrous Powder from Bovine Tendon, BSE-Free
Product Information
BUGA-CoL Type I Atelocollagen Insoluble Fibrous Powder from Bovine Tendon (BUGA-AteloCol-FB), BSE-free, is a product manufactured to medical-grade purity, setting the standard for all collagens in terms of purity (>99.9% collagen content), functionality, and natural collagen properties.
BUGA-AteloCol-FB undergoes FDA-approved viral inactivation studies. The raw materials are sourced from countries free from TSE/BSE, and production is carried out in compliance with TSE EN ISO 22442 1-2-3 standards. Certification of analysis, confirming the absence of TSE/BSE residues, is provided using test methods from the European Pharmacopoeia (EP 2.6.14 Method C) and the United States Pharmacopoeia (USP <85>).
BUGA-AteloCol-FB contains insoluble atelocollagen fibers, composed of approximately 97% Type I collagen, with the remainder being Type III collagen. The product is supplied in a lyophilized fibrous form and can be dispersed in 0.01 N hydrochloric acid or 0.5 M acetic acid.
• Weigh the amount of fibrous collagen (BUGA-AteloCol-FB) needed for your work.
• Prepare the solution according to the concentration you are working with (0.01 N hydrochloric acid or 0.5 N acetic acid).
• Continuously mix for at least 15 minutes until the collagen is fully wetted and the suspension appears semi-solution-like (using a magnetic, mechanical stirrer, or pipetting).
• Once fully mixed, the solution's pH value should be in the range of 2-3.
• Use a homogenizer or equivalent to ensure the collagen is completely homogeneous. Make sure the mixture temperature does not exceed 24°C. Once completed, the viscous suspension should contain very little visible solid material. Air bubbles may be common.
• To work at lower concentrations, dilute the solution with an appropriate acid solution and store unused portions at +4°C.
• Biomaterials
• Tissue scaffolds
• Foams
• Sponges
• Suspensions
• Coatings
• Pastes
• Films
• Sheets
Technical Specifications of the Product
| Appearance | Lyophilized Fibrous Powder | |
Smell | No Data Available | |
Extraction Method | Enzymatic - Atelocollagen | |
Sterilization Method | Filtration | |
Solubility/Collagen Concentration | 1, 3, 6 mg/ml (0,01 M HCl ) | |
Storage Temperature | Store at -20 ºC. Once reconstituted, store at 2-8 ºC. | |
Shelf Life | 2 years from the date of manufacture | |
Solution Shelf Life | 3 months after reconstitution (+4 ºC) | |
Packaging Quantity | 1 g, 5 g, 10 g | |
Collagen Purity - Silver Staining | ~98,9 | |
Source (Animal Species) | Bovine tendon/Flexor tendon | |
Endotoxin/LAL European Pharmacopoeia (EP 2.6.14 Method C) United States Pharmacopoeia (USP <85>) | <0,2 EU/ml | |
Microbiological Analysis United States Pharmacopoeia (USP <61>) | Aerobic Mesophilic Bacterial CFU/ml | <10 |
Anaerobic Mesophilic Bacteria (30°C) CFU/ml | <10 | |
Fungi-Yeast CFU/ml | <10 | |
SDS PAGE (Molecular Weight and Impurity Determination) | α-1 and α-2 chains with a molecular weight of 125-150 kDa, and β and γ chains above 275 kDa are observed. | |
• Divakar, P., Yin, K., & Wegst, U. G. (2019). Values and property charts for anisotropic freeze-cast collagen scaffolds for tissue regeneration. Data in brief, 22, 502-507.
• Divakar, P., Yin, K., & Wegst, U. G. (2019). Anisotropic freeze-cast collagen scaffolds for tissue regeneration: How processing conditions affect structure and properties in the dry and fully hydrated states. Journal of the mechanical behavior of biomedical materials, 90, 350-364.
• Kim, S. A., Sur, Y. J., Cho, M. L., Go, E. J., Kim, Y. H., Shetty, A. A., & Kim, S. J. (2020). Atelocollagen promotes chondrogenic differentiation of human adipose-derived mesenchymal stem cells. Scientific Reports, 10(1), 10678.
