BUGA-CoL Type I Telocollagen Solutions from Bovine Tendon, Sterile Filtrated, BSE Free
Product Info
BUGA-CoL Type I Telocollagen Solutions from Bovine Tendon, Sterile Filtrated, BSE Free (BUGA-TeloCol Solutions) is a medical grade product and is the standard for all collagens in terms of purity (> 99.9% collagen content), functionality and existing native collagen. BUGA-TeloCol Solutions is subject to FDA approved viral inactivation studies. TSE/BSE
Production is carried out within the scope of TSE EN ISO 22442 1-2-3 standards by supplying raw materials from countries that do not have this status. The certificate of analysis stating that there is no TSE/BSE residue is issued with the European Pharmacopoeia (EP 2.6.14 Method C) and American Pharmacopoeia (USP<85>) test methods.
BUGA-TeloCol Solutions consist of approximately 97% Type I collagen and 3% Type III collagen. These products are supplied in 3 mg/ml, 6 mg/ml and 10 mg/ml concentrations. Each product is shipped in a package containing a pre-formulated neutralizing buffer solution for the formation of collagen gel and 50 ml of collagen solution. BUGA-TeloCol Solutions are filtered through filters with appropriate pore sizes to be free of BSE and are presented to you in a sterile state. Aseptic practices should be used to maintain the sterility of the product during the preparation and processing of collagen and other solutions.
• Cell Culture Studies
• Surface Coatings
• Biopolymer Applications
• Material Engineering
• Hydrogel Studies
• For research and development purposes only.
• BUGA-TeloCol Solutions are in solution form and are ready-to-use sterile products for your formulation and experimental studies.
• If necessary, you can dilute the solution to the desired concentration using hydrochloric acid or acetic acid. After dilution, mix until a homogeneous appearance is obtained.
• You can store the solution product at +4 °C for up to 3 months in a stable condition.
Product Technical Specifications
Appearance | Lyophilized white color | |
Smell | No data available | |
Extraction method | Acidic-Telocollagen | |
Sterilization method | Filtration | |
Solubility/Collagen concentration | 1, 3, 6 mg/ml (in 0.01 M HCl) | |
Storage temperature | Store at -20 ºC. After solution, store at 2-8 ºC. | |
Shelf life | 2 years from date of production | |
Solution shelf life | After making it into solution, 3 months (at +4 ºC) | |
Collagen purity - Silver staining | ~98,9 | |
Source (Animal type) | Beef tendon | |
Endotoxin/LAL European Pharmacopoeia (EP 2.6.14 Method C) American Pharmacopoeia (USP<85>) | <0,5 EU/ml | |
Microbiological Analysis American Pharmacopoeia (USP<61>) | Aerobic Mesophilic Bacteria cfu/ml | <10 |
Anaerobic Mesophilic Bacteria (30 ℃) cfu/ml | <10 | |
Mold-Yeast cfu/ml | <10 | |
References
• Slyker, L., Diamantides, N., Kim, J., & Bonassar, L. J. (2022). Mechanical performance of collagen gels is dependent on purity, α1/α2 ratio, and telopeptides. Journal of Biomedical Materials Research Part A, 110(1), 11-20.
• Drzewiecki, K. E., Grisham, D. R., Parmar, A. S., Nanda, V., & Shreiber, D. I. (2016). Circular dichroism spectroscopy of collagen fibrillogenesis: A new use for an old technique. Biophysical journal, 111(11), 2377-2386.
• Wingender, B., Bradley, P., Saxena, N., Ruberti, J. W., & Gower, L. (2016). Biomimetic organization of collagen matrices to template bone-like microstructures. Matrix Biology, 52, 384-396.
• Burla, F., Tauber, J., Dussi, S., van Der Gucht, J., & Koenderink, G. H. (2019). Stress management in composite biopolymer networks. Nature physics, 15(6), 549-553.
• Paten, J. A., Siadat, S. M., Susilo, M. E., Ismail, E. N., Stoner, J. L., Rothstein, J. P., & Ruberti, J. W. (2016). Flow-induced crystallization of collagen: a potentially critical mechanism in early tissue formation. Acs Nano, 10(5), 5027-5040.
• Mull, V. (2023). Development of Adhesion Force microscopy for the surface charge analysis of collagen fibrils in vivo and in vitro.
