BUGA-CoL Type I Atelocollagen Solutions for Cell Culture (in PBS) Sterile Filtrated BSE Free 6mg/ml
Product Info
BUGA-CoL Type I Atelocollagen Solutions for Cell Culture (in PBS), Sterile Filtrated, BSE Free, 6 mg/ml (BUGA-Atelocol Solutions-CC) is a product manufactured to medical-grade purity, setting the standard for all collagens in terms of purity (>99.9% collagen content), functionality, and natural collagen properties. BUGA-Atelocol Solutions-CC undergoes FDA-approved viral inactivation studies. The raw materials are sourced from countries free from TSE/BSE, and production is carried out in compliance with TSE EN ISO 22442 1-2-3 standards. The certificate of analysis confirming the absence of TSE/BSE residues is provided using European Pharmacopoeia (EP 2.6.14 Method C) and United States Pharmacopoeia (USP <85>) test methods. BUGA-Atelocol Solutions-CC is composed of approximately 97% Type I collagen, with the remaining 3% Type III collagen. BUGA-Atelocol Solutions-CC is sterile-filtered using filters with appropriate pore sizes to ensure the absence of BSE. BUGA-Atelocol Solutions-CC can be directly used for cell culture, preparation of thin layers, or use as a solid gel in applications.
• Atelokolajenin 6 mg/ml konsantrasyonunda PBS ortamında hazırlanmış çözeltisi kullanıma hazır üründür.
• Gerek hücre kültürü uygulamalarında gerekse doku iskelesi tasarımında belirlediğiniz derişime PBS ile seyrelterek kullanabilirsiniz.
• Uzun süreli kullanılmama durumunda +4 °C’de saklayınız.
• Çözelti ürünü vorteksleme, hızlı çalkalama yapmayınız. Dondurma ve tekrar çözüp kullanma işlemi çapraz bağlanmaya neden olacağından önerilmez.
• Hücre Kültürü
• Doku İskelesi Tasarımları
• 3d Biyomalzemeler
• Biyomühendislik
• Malzeme Bilimi
• Biyoteknoloji
• Nanoteknoloji
Technical Specifications of the Product
Appearance | Intense white color | |
Smell | No Data Available | |
Extraction Method | Enzymatic-Atelocollagen (in PBS) | |
Sterilization Method | Filtration | |
Solubility/Collagen Concentration | 6 mg/ml (in PBS ) | |
Storage Temperature | 2-8 ºC | |
Shelf Life | 3 months from the date of manufacture | |
Packaging Quantity | 100 ml- 1000ml | |
Source (Animal Species) | Bovine Tendon | |
Endotoxin/LAL European Pharmacopoeia (EP 2.6.14 Method C) United States Pharmacopoeia (USP <85>) | <0,1 EU/ml | |
Microbiological Analysis United States Pharmacopoeia (USP <61>) | Aerobic Mesophilic Bacterial CFU/ml | <10 |
Anaerobic Mesophilic Bacteria (30°C) CFU/ml | <10 | |
Fungi-Yeast CFU/ml | <10 | |
SDS PAGE (Molecular Weight and Impurity Determination) | α-1 and α-2 chains with a molecular weight of 125-150 kDa, and β and γ chains above 275 kDa are observed. | |
• Gehwolf, R., Spitzer, G., Wagner, A., Lehner, C., Weissenbacher, N., Tempfer, H., & Traweger, A. (2019). 3D-embedded cell cultures to study tendon biology. Stem Cells and Aging: Methods and Protocols, 155-165.
• Rahmanian, M., & Heldin, P. (2002). Testicular hyaluronidase induces tubular structures of endothelial cells grown in three‐dimensional colagen gel through a CD44‐mediated mechanism. International journal of cancer, 97(5), 601-607.
• Szász, C., Pap, D., Szebeni, B., Bokrossy, P., Őrfi, L., Szabó, A. J., ... & Veres-Székely, A. (2023). Optimization of Sirius Red-Based Microplate Assay to Investigate Collagen Production In Vitro. International Journal of Molecular Sciences, 24(24), 17435.
• Song, Y., Zhang, Y., Qu, Q., Zhang, X., Lu, T., Xu, J., ... & Xiong, R. (2023). Biomaterials based on hyaluronic acid, collagen and peptides for three-dimensional cell culture and their application in stem cell differentiation. International Journal of Biological Macromolecules, 226, 14-36.
• Bouhlel, W., Kui, J., Bibette, J., & Bremond, N. (2022). Encapsulation of cells in a collagen matrix surrounded by an alginate hydrogel shell for 3D cell culture. ACS Biomaterials Science & Engineering, 8(6), 2700-2708.
• Iwamoto, Y., Haraguchi, R., Nakao, R., Aoki, S., Oishi, Y., & Narita, T. (2022). One-Pot Preparation of Collagen Tubes Using Diffusing Gelation. ACS omega, 7(26), 22872-22878.
• Besseau, L., Coulomb, B., Lebreton-Decoster, C., & Giraud-Guille, M. M. (2002). Production of ordered collagen matrices for three-dimensional cell culture. Biomaterials, 23(1), 27-36.
• Sung, K. E., Su, G., Pehlke, C., Trier, S. M., Eliceiri, K. W., Keely, P. J., ... & Beebe, D. J. (2009). Control of 3-dimensional collagen matrix polymerization for reproducible human mammary fibroblast cell culture in microfluidic devices. Biomaterials, 30(27), 4833-4841.
• Themistocleous, G. S., Katopodis, H., Sourla, A., Lembessis, P., Doillon, C. J., Soucacos, P. N., & Koutsilieris, M. (2004). Three-dimensional type I collagen cell culture systems for the study of bone pathophysiology. in vivo, 18(6), 687-696.
• Artym, V. V., & Matsumoto, K. (2010). Imaging cells in three‐dimensional collagen matrix. Current protocols in cell biology, 48(1), 10-18.
