• Weigh the desired amount of BUGA-TeloCol product and/or determine its concentration, then transfer it to an appropriately sized container.
• Prepare a 0.01 M hydrochloric acid or 0.5 M acetic acid solution suitable for the working volume after weighing the lyophilized product.
• Dissolve the lyophilized product and the prepared acid solution in an appropriate mixing container (borosilicate beaker or bottle, etc.) with a magnetic stirrer at a low rpm (preferably 350-500 rpm) until fully dissolved. You can also use pipetting. Depending on the concentration, gelation may occur, which can be handled by intensive pipetting.
• If the prepared solution will not be used immediately, store it in a refrigerator at +4 °C.
• Note: Freezing the dissolved product is not recommended.
• Important Note: Avoid rapid stirring, shaking, or vortexing after forming the solution as it may cause cross-linking in the product.

Coating Procedure
• Prepare the BUGA-TeloCol solution as described above. If necessary, dilute to the desired volume. Dilutions should be performed with the same acid solution used for preparation (0.01 N hydrochloric acid or 0.5 N acetic acid). Mix until a homogeneous appearance is achieved after dilution.
• Add an appropriate amount of diluted BUGA-TeloCol to the culture surface, ensuring full surface coverage.
• Incubate at room temperature in a covered environment for 1-2 hours. Aspirate any remaining material or let it incubate until the surface dries.
• Carefully rinse the coated surfaces in a sterile environment or with PBS to avoid surface scratches.
• Coated surfaces are ready for use and can be stored at 2-10°C under sterile conditions.

3-D Gel Preparation Procedure (With Neutralization Solution)
Note: During collagen preparation, it is recommended to cool the collagen and other working solutions and keep them on ice.
• Determine the desired collagen amount and prepare the BUGA-TeloCol solution as described above.
• Transfer one part of the chilled neutralization solution into a sterile mixing container or tube.
• Slowly mix the collagen with the neutralization solution.
• Gently swirl or use pipetting for mixing (vortexing is not recommended).
• Distribute the collagen mixture into desired sterile plates or culture dishes.
• Incubate at 37°C for 1 hour to form the gel. 3-D Gel Preparation Procedure (Without Neutralization Solution)
• Gently mix chilled 10x PBS or 10x culture medium with the collagen solution.
• Adjust the pH of the mixture to 7.2-7.6 using sterile 0.1 M sodium hydroxide. Carefully monitor the pH (using a pH meter, phenol red, or pH paper) and mix slowly.
• Adjust the final volume to a total of 10 parts with sterile water and mix.
• Keep the mixture at 2-8°C to prevent gelation.
• Distribute the BUGA-Telocollagen mixture into desired sterile plates or culture dishes.
• Heat to 37°C to initiate gel formation and incubate for approximately 30 to 60 minutes.

• Cell culture studies
• Surface coatings
• Biopolymer applications
• Material engineering
• Suitable for hydrogel studies
• For research and development purposes only.

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